biotae, Astyanax Castro [R. M. C.] & Vari [R. P.] 2004:331, Fig. 1 [Proceedings of the Biological Society of Washington v. 117 (no. 3); ref. 27972] Upper rio Paraná system, Rio Paranapanema basin, Município de Diamante do Norte, Fazenda Água Mole, 22°38'31.7"S, 52°48'59.0"W, Paraná State, Brazil. Holotype: LIRP 4009. Paratypes: LIRP 2734 (15), 4021 (2 c&s), 4276 (34); MZUSP 79807 (10); USNM 373492 (15). •Valid as Astyanax biotae Castro & Vari 2004 -- (Protogino et al. 2006:11 [ref. 28780], Oliveira et al. 2017:179 [ref. 35587], da Graça et al. 2017:12 [ref. 35556], Reis et al. 2020:458 [ref. 38364]). Current status: Valid as Astyanax biotae Castro & Vari 2004. Characidae: Stethaprioninae. Distribution: South America: upper Paraná River system (southeastern Brazil). Habitat: freshwater.